Tg(EEF1A1-Ahr)1Pjvt
Transgene Detail
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Symbol: |
Tg(EEF1A1-Ahr)1Pjvt |
Name: |
transgene insertion 1, Raimo Pohjanvirta |
MGI ID: |
MGI:5475502 |
Synonyms: |
rWT, Tg(EF1alfa-Ahr), Tg WT-rat AH receptor |
Transgene: |
Tg(EEF1A1-Ahr)1Pjvt Location: unknown
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Alliance: |
Tg(EEF1A1-Ahr)1Pjvt page
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Transgene Type: |
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Transgenic (Inserted expressed sequence) |
Mutation: |
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Insertion
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Tg(EEF1A1-Ahr)1Pjvt expresses
1 gene
Transgene expresses:
Organism |
Expressed Gene |
Homolog in Mouse |
Note |
rat |
Ahr (25690) |
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Mutation details: Mice bearing this transgene express the wild-type (Long-Evans) rat aryl hydrocarbon receptor. The transgene contains the promoter of the human eukaryotic translation elongation factor-1alpha1 (EEF1A1, also called "EF1alpha") followed by a 200 bp intron, a ~90 bp fusion of most of the mouse Ahr leader sequence with the 30-bp UTR of the rat Ahr mRNA, a cDNA encoding the wild-type rat AHR, the SV40 late polyadenylation signal, and nearly 800 bp of rat Ahr 3' UTR; this entire construct is flanked 5' by a 600 bp and 3' by a 250 bp chicken beta-globin core insulator. It was estimated by real-time quantitative polymerase chain reaction (qPCR) analysis that 5 copies of the transgene are present per haploid genome. Transgene-derived mRNA is present in every one of multiple tissues analyzed by reverse transcription-PCR (RT-PCR) and has been shown to be inducible by orally administered 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in the liver, where the rat protein also has been detected by western blot analysis.
(J:147952)
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 1 strain available
Cell Lines: 0 lines available
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Original: |
J:147952 Pohjanvirta R, Transgenic mouse lines expressing rat AH receptor variants--a new animal model for research on AH receptor function and dioxin toxicity mechanisms. Toxicol Appl Pharmacol. 2009 Apr 15;236(2):166-82 |
All: |
1 reference(s) |
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