Pdgfra^{tm1.1(cre/Esr1*)Nshk}
Targeted Allele Detail

Summary

• Symbol: Pdgfra^{tm1.1(cre/Esr1*)Nshk}
• Name: platelet derived growth factor receptor, alpha polypeptide; targeted mutation 1, Shin-Ichi Nishikawa
• MGI ID: MGI:5475226
• Synonyms: PRalpha-MCM
• Gene: Pdgfra Location: Chr5:75312953-75358876 bp, + strand Genetic Position: Chr5, 39.55 cM
• Alliance: Pdgfra^{tm1.1(cre/Esr1*)Nshk} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:193389
• Parent Cell Line: TT2 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Pdgfra^{tm1.1(cre/Esr1*)Nshk} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Pdgfra (MGI:97530)

• Mutation details: A targeting vector was constructed with Pdgfra homology arms, the tamoxifen-inducible MerCreMer cDNA with an adenoviral splice acceptor sequence (SA) added to the 5' side of the MCM cDNA to prevent cryptic splicing and an Frt-flanked neo cassette 3' to the the MCM sequence. This construct was injected into ES cells. Flp excised the neo cassette from the final allele. (J:193389)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Pdgfra (mouse) Summary of all recombinase alleles driven by Pdgfra. Alliance mouse Pdgfra gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pdgfra Mutation: 85 strains or lines available

References

• Original: J:193389 Ding G, et al., PDGF Receptor Alpha+ Mesoderm Contributes to Endothelial and Hematopoietic Cells in Mice. Dev Dyn. 2013 Mar;242(3):254-68
• All: 5 reference(s)