H3f3b^tm1.1Psk
Targeted Allele Detail

Summary

• Symbol: H3f3b^tm1.1Psk
• Name: H3.3 histone B; targeted mutation 1.1, Paul Knoepfler
• MGI ID: MGI:5474462
• Synonyms: H3f3b^delta
• Gene: H3f3b Location: Chr11:115912738-115918788 bp, - strand Genetic Position: Chr11, 80.91 cM, cytoband E2
• Alliance: H3f3b^tm1.1Psk page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:194593
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: H3f3b^tm1Psk mutant mice were bred to oocyte-specific Tg(Zp3-cre)3Mrt Cre deleter mice to excise exons 2-4. Southern blot, qPCR on RNA and chromatin immunoprecipitation-sequencing confirmed the null allele. (J:194593)

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any H3f3b Mutation: 14 strains or lines available

References

• Original: J:194593 Bush KM, et al., Endogenous mammalian histone H3.3 exhibits chromatin-related functions during development. Epigenetics Chromatin. 2013 Apr 9;6(1):7
• All: 2 reference(s)