Cbfb^tm5Ppl
Targeted Allele Detail

Summary

• Symbol: Cbfb^tm5Ppl
• Name: core binding factor beta; targeted mutation 5, Pu P Liu
• MGI ID: MGI:5469976
• Synonyms: Cbfb^deltaC95, Cbfb-MYH11deltaC95
• Gene: Cbfb Location: Chr8:105897306-105944621 bp, + strand Genetic Position: Chr8, 53.04 cM
• Alliance: Cbfb^tm5Ppl page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:193140
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Humanized sequence, Inserted expressed sequence, Null/knockout)
• Mutation: Insertion
• Mutation details: Exon 5 was replaced by a construct composed of the first 25 bp of mouse exon 5 fused to the 3' end of a human CBFB/MYH11 cDNA. This fusion protein is produced by a chromosome 16 inversion in patients with acute myeloid leukemia subtype M4Eo. The human cDNA fragment used contained the last 66 bp of human exon 5 and exons 33-42 of MYH11 lacking the sequence encoding amino acids the last 95 amino acids (deltaC95). A bovine growth hormone polyadenylation site and a floxed PGK-neomycin cassette were inserted immediately downstream. (J:193140)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cbfb Mutation: 39 strains or lines available

References

• Original: J:193140 Kamikubo Y, et al., The C-terminus of CBFbeta-SMMHC is required to induce embryonic hematopoietic defects and leukemogenesis. Blood. 2013 Jan 24;121(4):638-42
• All: 1 reference(s)