Mrgprb4^tm3(cre)And
Targeted Allele Detail

Summary

• Symbol: Mrgprb4^tm3(cre)And
• Name: MAS-related GPR, member B4; targeted mutation 3, David J Anderson
• MGI ID: MGI:5448562
• Synonyms: Mrgprb4-cre, MrgprB4-mtdTomato-2A-NLScre-frt-PGK-neo-frt
• Gene: Mrgprb4 Location: Chr7:47847961-47848926 bp, - strand Genetic Position: Chr7, 31.11 cM
• Alliance: Mrgprb4^tm3(cre)And page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:194547
• Parent Cell Line: CJ7 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutations: Insertion, Intragenic deletion
• Mrgprb4^tm3(cre)And expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Mrgprb4 (MGI:3033119)

• Mutation details: The entire open reading frame, encoded by a single exon, was replaced with an mtdTomato-2A-NLSCre-Frt-PGK-Neomycin-FRT cassette. The membrane-tagged tdTomato (mtdTomato) contains the 8 amino acids of the MARCKS sequence (MGCCFSKT) fused to the N-terminus of the full-length tdTomato and was connected to a nuclear localization signal (NLS)-tagged Cre recombinase via an intervening foot-and-mouth disease virus 2A peptide (FMDV 2A or F2A) sequence. (J:194547)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Mrgprb4 (mouse) Summary of all recombinase alleles driven by Mrgprb4. Alliance mouse Mrgprb4 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Mrgprb4 Mutation: 25 strains or lines available

References

• Original: J:194547 Vrontou S, et al., Genetic identification of C fibres that detect massage-like stroking of hairy skin in vivo. Nature. 2013 Jan 31;493(7434):669-73
• All: 8 reference(s)