Tg(Th-Twnk*,-EGFP)2Gcor
Transgene Detail

Summary

• Symbol: Tg(Th-Twnk*,-EGFP)2Gcor
• Name: transgene insertion 2, Gino A Cortopassi
• MGI ID: MGI:5447072
• Synonyms: Tg(Th-Peo1*,-EGFP)2Gcor
• Transgene: Tg(Th-Twnk*,-EGFP)2Gcor Location: unknown
• Alliance: Tg(Th-Twnk*,-EGFP)2Gcor page

Transgene origin

• Strain of Origin: Not Specified

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Th-Twnk*,-EGFP)2Gcor expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Twnk (MGI:2137410)

• Tg(Th-Twnk*,-EGFP)2Gcor expression driven by 1 gene
  Transgene expression driven by:
  

  Organism

  Driver Gene

  Homolog in Mouse

  Note

  rat

  Th (25085)

  Th	(MGI:98735)

• Mutation details: The transgene contains 9.0 kb of upstream sequence from the rat tyrosine hydroxylase gene that drives dopamine neuron-specific expression of the mouse gene with a silent point mutation at position 1077 (c.1077C->G). This mutation forms a restriction enzyme site and allows for the insertion of 39 nucleotides that produces a duplication of amino acids 353 to 365. An IRES-EGFP and polyadenylation sequence follow the altered mouse gene. Four of six lines have higher EGFP expression than wild-type mice. Lines 1, 2, 3 and 4 contain 181, 227, 141, 97, 42 and 6 copies of the transgene, respectively. (J:188914)

Disease models

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:188914 Song L, et al., Mutant Twinkle increases dopaminergic neurodegeneration, mtDNA deletions and modulates Parkin expression. Hum Mol Genet. 2012 Dec 1;21(23):5147-58
• All: 2 reference(s)