Tg(Ada*-cre)5Xiay
Transgene Detail

Summary

• Symbol: Tg(Ada*-cre)5Xiay
• Name: transgene insertion 5, Yang Xia
• MGI ID: MGI:5444884
• Synonyms: Tpbpar/Adaf-AdaP-Cre
• Transgene: Tg(Ada*-cre)5Xiay Location: unknown
• Alliance: Tg(Ada*-cre)5Xiay page

Transgene origin

• Strain of Origin: FVB

Transgene description

• Transgene Type: Transgenic (Recombinase)
• Mutation: Insertion
• Tg(Ada*-cre)5Xiay expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Tpbpa (MGI:98795)

• Mutation details: A construct was generated with a Tpbpa/Ada chimeric enhancer and Ada gene basal promoter ligated to the cre cDNA containing a nuclear localization signal (NLS). The 0.4 kb trophoblast specific protein alpha enhancer was cloned in reverse orientation (Tpbpar) with the 1.8 kb adenosine deaminase enhancer in forward orientation (Adaf); this chimeric enhancer was combined with the 0.8 kb basal Ada promoter (AdaP) in the transgenic vector . The linearized construct was injected into pronuclei of FVB zygotes. Six founders were obtained and all showed similar transgene expression patterns. Data from the mouse with the highest transgene copy number (line 5) is presented in the original reference. (J:185323)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Tpbpa (mouse) Summary of all recombinase alleles driven by Tpbpa. Alliance mouse Tpbpa gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:185323 Zhou CC, et al., Targeted expression of Cre recombinase provokes placental-specific DNA recombination in transgenic mice. PLoS One. 2012;7(2):e29236
• All: 3 reference(s)