Ngb^tm1.2Geno
Targeted Allele Detail

Summary

• Symbol: Ngb^tm1.2Geno
• Name: neuroglobin; targeted mutation 1.2, Genoway
• MGI ID: MGI:5440446
• Gene: Ngb Location: Chr12:87144305-87149313 bp, - strand Genetic Position: Chr12, 41.29 cM, cytoband D3
• Alliance: Ngb^tm1.2Geno page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:188398
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was inserted upstrea of exon 2 and an FRT flanked neo cassette and loxP site were introduced downstream of exon 3 via homologous recombination. Recombinase mediated recombination removed exons 2 and 3 and the neo cassette. RT-QPCR, IHC and western blot analyses confirmed the absence of mRNA and protein expression in homozygous mice. (J:187107, J:188398)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ngb Mutation: 10 strains or lines available

References

• Original: J:188398 Hundahl CA, et al., Neuroglobin-deficiency exacerbates Hif1A and c-FOS response, but does not affect neuronal survival during severe hypoxia in vivo. PLoS One. 2011;6(12):e28160
• All: 7 reference(s)