Kdm6a^{tm1a(EUCOMM)Jhha}
Targeted Allele Detail

Summary

• Symbol: Kdm6a^{tm1a(EUCOMM)Jhha}
• Name: lysine (K)-specific demethylase 6A; targeted mutation 1a, Jacob H Hanna
• MGI ID: MGI:5438704
• Synonyms: Utx^flox
• Gene: Kdm6a Location: ChrX:18028814-18146175 bp, + strand Genetic Position: ChrX, 13.45 cM
• Alliance: Kdm6a^{tm1a(EUCOMM)Jhha} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:186686
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Conditional ready, Hypomorph, Reporter)
• Mutation: Insertion
• Mutation details: The targeting vector from EUCOMM was used to insert an FRT flanked lacZ and neo cassette and a loxP site upstream of exon 3 and a second loxP site downstream of exon 3 via homologous recombination. Slightly reduced levels of mRNA expression are seen in hemizygous male embryonic stem cells carrying this allele indicating that this is a hypomorphic allele. (J:186686)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Kdm6a Mutation: 38 strains or lines available

Notes

In J:186686, the targeting construct was injected into V6.5 ES cells [(C57BL/6 x 129S4/SvJae)F1], V19-OSKM ES cells [(C57BL/6 x 129S4/SvJae)F1], and BVSC ES cells [(C57BL/6 x DBA)F2].

References

• Original: J:186686 Mansour AA, et al., The H3K27 demethylase Utx regulates somatic and germ cell epigenetic reprogramming. Nature. 2012 Aug 16;488(7411):409-13
• All: 1 reference(s)