Tg(ACTA1-cre/Esr1*)2Kesr
Transgene Detail

Summary

• Symbol: Tg(ACTA1-cre/Esr1*)2Kesr
• Name: transgene insertion 2, Karyn A Esser
• MGI ID: MGI:5435875
• Synonyms: HSA-Cre, HSA-MCM, HSA-MerCreMer
• Transgene: Tg(ACTA1-cre/Esr1*)2Kesr Location: unknown
• Alliance: Tg(ACTA1-cre/Esr1*)2Kesr page

Transgene origin

• Strain of Origin: (C57BL/6 x C3H)F2

Transgene description

• Transgene Type: Transgenic (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Tg(ACTA1-cre/Esr1*)2Kesr expression driven by 1 gene
  Transgene expression driven by:
  

  Organism

  Driver Gene

  Homolog in Mouse

  Note

  human

  ACTA1 (58)

  Acta1

  (MGI:87902)

• Mutation details: The human alpha-skeletal actin gene promoter (ACTA1; HSA) was cloned into a vector upstream the cre recombinase gene fused at the N- and C-termini to mutated estrogen receptor ligand-binding domains (MerCreMer). A beta-globin intron II sequence followed by a polyadenylation sequence was inserted downstream of the promoter to ensure proper splicing. The construct was injected into B6C3F2 embryos. Five founders were generated and crossed to Rosa-lacz reporter mice to assess germline transmission and inducible muscle-specific recombinase activity. Line 2 was established. (J:186782)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	ACTA1 (human) Summary of all recombinase alleles driven by ACTA1. Comparative matrix view of recombinase activities Alliance human ACTA1 gene page

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available

References

• Original: J:186782 McCarthy JJ, et al., Inducible Cre transgenic mouse strain for skeletal muscle-specific gene targeting. Skelet Muscle. 2012;2(1):8
• All: 70 reference(s)