Tg(Wnt1-flpe/ERT2)9455Dym
Transgene Detail

Summary

• Symbol: Tg(Wnt1-flpe/ERT2)9455Dym
• Name: transgene insertion 9455, Susan Dymecki
• MGI ID: MGI:5432117
• Synonyms: Wnt1::FlpeER^T2
• Transgene: Tg(Wnt1-flpe/ERT2)9455Dym Location: unknown
• Alliance: Tg(Wnt1-flpe/ERT2)9455Dym page

Transgene origin

• Strain of Origin: (C57BL/6 x SJL)F2 or FVB

Transgene description

• Transgene Type: Transgenic (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Tg(Wnt1-flpe/ERT2)9455Dym expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  Not Specified	Wnt1

• Mutation details: The construct was constructed by inserting a cassette containing the flpe/ERT2 coding and polyA sequences into a Wnt1 expression-vector. 11 kb of Wnt1 genomic DNA containing critical 3' enhancer sequences were included after the FLPe/ERT2 cassette. Tandem insulator sequences flanked the complete construct. The purified transgene was injected into fertilized eggs to produce transgenic animals. 11 lines were obtained and FLPe/ERT2 mRNA expression recapitulated endogenous Wnt1 expression. Two lines showing the highest levels of FLPe/ERT2 mRNA were characterized with respect to recombinase activity. Data from line 9455 was shown in detail. (J:97320)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Wnt1 (species not specified) Summary of all recombinase alleles driven by Wnt1.

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:97320 Hunter NL, et al., Ligand-activated Flpe for temporally regulated gene modifications. Genesis. 2005 Mar;41(3):99-109
• All: 1 reference(s)