Trp53^tm1.1Rfo
Targeted Allele Detail

Summary

• Symbol: Trp53^tm1.1Rfo
• Name: transformation related protein 53; targeted mutation 1.1, Riccardo Fodde
• MGI ID: MGI:5431904
• Synonyms: p53^P275S
• Gene: Trp53 Location: Chr11:69471185-69482699 bp, + strand Genetic Position: Chr11, 42.83 cM, cytoband B2-C
• Alliance: Trp53^tm1.1Rfo page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:186114
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Humanized sequence)
• Mutations: Insertion, Single point mutation
• Mutation details: A loxP site flanked neomycin resistance gene and STOP cassette was inserted into intron 1. Exon 8 was replaced with a modified exon 8 in which a C-to-T substitution results in the amino acid substitution of proline with serine at position 275 (p.P275S). This mutation corresponds to a proven UVB exposure mutational hostspot at human codon 278. Cre-mediated recombination removed the neo/STOP cassette. (J:186114)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Trp53 Mutation: 232 strains or lines available

References

• Original: J:186114 Voskamp P, et al., Rapamycin impairs UV induction of mutant-p53 overexpressing cell clusters without affecting tumor onset. Int J Cancer. 2012 Sep 15;131(6):1267-76
• All: 1 reference(s)