Opn1sw^tm1.1Pugh
Targeted Allele Detail

Summary

• Symbol: Opn1sw^tm1.1Pugh
• Name: opsin 1 (cone pigments), short-wave-sensitive (color blindness, tritan); targeted mutation 1.1, Edward Pugh
• MGI ID: MGI:5431113
• Synonyms: Opn1sw^F81Y
• Gene: Opn1sw Location: Chr6:29376670-29380512 bp, - strand Genetic Position: Chr6, 12.36 cM
• Alliance: Opn1sw^tm1.1Pugh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:185275
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutations: Insertion, Single point mutation
• Mutation details: Exon 1 was replaced with one in which an A to T transition results in the amino acid substitution of tyrosine for phenylalanine at position 81 (F81Y). An FRT-flanked neo cassette was inserted downstream of exon 3. Flp-mediated recombination removed the neo cassette. Western blot analysis confirmed a 2-fold reduction in protein expression. (J:185275)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Opn1sw Mutation: 23 strains or lines available

References

• Original: J:185275 Insinna C, et al., An S-Opsin Knock-In Mouse (F81Y) Reveals a Role for the Native Ligand 11-cis-Retinal in Cone Opsin Biosynthesis. J Neurosci. 2012 Jun 6;32(23):8094-104
• All: 1 reference(s)