Tg(Msx3-icre)1/1Wdr
Transgene Detail

Summary

• Symbol: Tg(Msx3-icre)1/1Wdr
• Name: transgene insertion 1/1, William D Richardson
• MGI ID: MGI:5430776
• Synonyms: line 1/1, Msx3-cre, Msx3/iCre
• Transgene: Tg(Msx3-icre)1/1Wdr Location: unknown
• Alliance: Tg(Msx3-icre)1/1Wdr page

Transgene origin

• Strain of Origin: Not Specified

Transgene description

• Transgene Type: Transgenic (Recombinase)
• Mutation: Insertion
• Tg(Msx3-icre)1/1Wdr expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  Not Specified	Msx3

• Mutation details: A targeting vector was used to insert a codon improved cre recombinase (icre) and an Frt-flanked chloramphenicol selection cassette into exon 1 of the Msx3 locus in a phage artificial chromosome (PAC 402-G09) by homologous recombination. This resulted in fusing the icre sequence to the ATG of Msx3. The selection cassette was removed by induction of Flpe in EL250 cells, and the purified vector was used for pronuclear injection. 8 founders were obtained. Of these 2 were studied in more detail (1/1 and 3/2), displaying identical patterns of cre expression and GFP expression when crossed to ROSA-GFP reporter mice. Line 1/1 was used for derivation of animals for experimental study. (J:186011)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Msx3 (species not specified) Summary of all recombinase alleles driven by Msx3.

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:186011 Fogarty MH, Fate-mapping the mouse neural tube by Cre-loxP transgenesis. PhD Thesis - University of London. 2006
• All: 4 reference(s)