Tg(Pklr-SV40)1Ak
Transgene Detail

Summary

• Symbol: Tg(Pklr-SV40)1Ak
• Name: transgene insertion 1, Axel Kahn
• MGI ID: MGI:5429578
• Synonyms: L-PK/Tag1
• Transgene: Tg(Pklr-SV40)1Ak Location: unknown
• Alliance: Tg(Pklr-SV40)1Ak page

Transgene origin

• Strain of Origin: (C57BL/6 x DBA)F1

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: This transgene consists of the SV40 early genes including the coding sequence of the transforming large tumor and small tumor antigens under the control of the rat L-type pyruvate kinase gene regulatory region. Transcription of Pklr is dependent on metabolic and hormonal controls such that glucose and insulin increase transcription while fasting, refeeding a low-carbohydrate diet and glucagon inhibit Pklr transcription. Thus manipulation of diet can result in expression of the transgene. Three lines were established with different transgene copy number (Lines 1,2,3). Line 1 carries approximately 100 copies of the transgene. Northern blot analysis indicates transgene expression in the liver and to a lesser degree in the small intestine, kidney, and pancreas. (J:185488)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:185488 Cartier N, et al., Diet-dependent carcinogenesis of pancreatic islets and liver in transgenic mice expressing oncogenes under the control of the L-type pyruvate kinase gene promoter. Oncogene. 1992 Jul;7(7):1413-22
• All: 1 reference(s)