Mir350^tm1Wtsi
Targeted Allele Detail

Summary

• Symbol: Mir350^tm1Wtsi
• Name: microRNA 350; targeted mutation 1, Wellcome Trust Sanger Institute
• MGI ID: MGI:5320061
• Synonyms: Mir350^{tm1(mirKO)Wtsi}
• Gene: Mir350 Location: Chr1:176599891-176599989 bp, - strand Genetic Position: Chr1, 82.65 cM
• Alliance: Mir350^tm1Wtsi page

Mutation origin

• Mutant Cell Lines: mirKO_ES_PuDtk_2G7, mirKO_ES_PuDtk_2G8, mirKO_ES_PuDtk_2G9, mirKO_ES_PuDtk_2G10, mirKO_ES_PuDtk_2G11, mirKO_ES_PuDtk_2G12
• Germline Transmission: Earliest citation of germline transmission: J:204812
• Parent Cell Line: JM8A3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: Sanger miRNA knockouts

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion Vector: PGK_EM7_PuDtk_bGHpA
• Mutation details: The insertion of the PGK_EM7_PuDtk_bGHpA cassette created a deletion of size 216 starting at position 176599829 and ending at position 176600045 of Chromosome 1 (Build GRCm39). This deletion eliminates the DNA sequence for this microRNA. The cassette is composed of a loxP site followed by an F3 site followed by a PGK-puromycin-delta-tk cassette, a loxP site and finally an FRT site. (J:174268)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mir350 Mutation: 1 strain or line available

References

• Original: J:174268 Prosser HM, et al., A resource of vectors and ES cells for targeted deletion of microRNAs in mice. Nat Biotechnol. 2011 Aug 7;29(9):840-5
• All: 3 reference(s)