Igs1^{tm5(CAG-Bgeo,-Mito-GFP)Nat}
Targeted Allele Detail

Summary

• Symbol: Igs1^{tm5(CAG-Bgeo,-Mito-GFP)Nat}
• Name: intergenic site 1; targeted mutation 5, Jeremy Nathans
• MGI ID: MGI:5319536
• Synonyms: Iis1^{tm5(CAG-Bgeo,-Mito-GFP)Nat}, Iis1^{tm5(CAG-Bgeo,-Mito-GFP/V5)Nat}, Ubb^{tm1(Mito-GFP-V5)Nat}
• Gene: Igs1 Location: unknown Genetic Position: Chr11, Syntenic
• Alliance: Igs1^{tm5(CAG-Bgeo,-Mito-GFP)Nat} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:101977
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change, Reporter)
• Mutation: Insertion
• Mutation details: The following elements were inserted, in order, 2 kb 5' of the Ubb gene by targeted recombination in 129-derived ES cells: CMV enhancer/beta-actin promoter (CAG), loxP site, beta-geo, 3 transcription sites, loxP site, mitochondria targeted GFP fused to a C-terminal V5 epitope, and a bovine growth hormone 3' UTR. Mitochondrial targeting was achieved by using the N-terminal 25 amino acid targeting signal of mouse Cox8a. The V5 epitope encodes amino acid residues 95-108 (GKPIPNPLLGLDST) of RNA polymerase alpha subunit of simian virus 5. (J:101977)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Igs1 Mutation: 10 strains or lines available

References

• Original: J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017
• All: 2 reference(s)