Gt(ROSA)26Sor^{tm9(ACTB-EGFP*,-tTA2,tetO-tdTomato)Luo}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm9(ACTB-EGFP*,-tTA2,tetO-tdTomato)Luo}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 9, Liqun Luo
• MGI ID: MGI:5314255
• Synonyms: R26^G-TET
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm9(ACTB-EGFP*,-tTA2,tetO-tdTomato)Luo} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:181830
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

Allele Type: Targeted (Inducible, Reporter, Transactivator) Inducer: doxycycline/tetracycline Mutation: Insertion Mutation details: A targeting vector was created with a CMV enhancer/chicken beta-actin core promoter (pCA) upstream of the MADM G-tTA2 cassette. The MADM G-tTA2 cassette used here (GFPN-terminus-intron-tTA2ATG-less) was designed with the N-terminal portion of a mutant enhanced green fluorescent protein (mut4EGFP first exon; nucleotides 1-274), a beta-globin intronic sequence (containing a frt-flanked region of two lox site variants [lox2272 and lox5171] and then a loxP-flanked neomycin resistance gene), a tetracycline responsive element (tetO), an ATG-less tdTomato sequence (nucleotides 3-1548 including stop codon) tagged with three copies of the Myc epitope at its C-terminus, and an SV40 T-antigen poly(A) signal. This entire construct was subcloned into the pROSA26-PA targeting vector to generate the final targeting construct pROSA26-G-TET. The pROSA26-G-TET construct was inserted into the Gt(ROSA)26Sor locus. (J:181830)

Generation of various Gt(ROSA)26Sor alleles

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1058 strains or lines available

References

• Original: J:181830 Tasic B, et al., Extensions of MADM (mosaic analysis with double markers) in mice. PLoS One. 2012;7(3):e33332
• All: 1 reference(s)