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Transgene Detail
Symbol: Tg(Phox2b-cre)1Jbr
Name: transgene insertion 1, Jean-Francois Brunet
MGI ID: MGI:5306618
Synonyms: Phox2b::Cre
Transgene: Tg(Phox2b-cre)1Jbr  Location: unknown  
Alliance: Tg(Phox2b-cre)1Jbr page
Strain of Origin:  C57BL/6 x DBA/2
Transgene Type:    Transgenic (Recombinase)
Mutation:    Insertion
Tg(Phox2b-cre)1Jbr expression driven by 1 gene
Mutation detailsThe cre coding sequence was inserted in-frame into exon 2 of the Phox2b gene in BAC RP24-95M11, so that a fusion protein contain the first 82 amino acids of Phox2b fused to cre recombinase will be produced. A two-step recombineering strategy was used with the first step resulting in the introduction of a dual antibiotic resistance cassette into exon 2 by homologous recombination and selection of positive clones, followed by a second round of recombination that exchanged the resistance cassette with one contain cre and an ampicillin resistance cassette. Correctly targeted BAC clones were further modified by removal of the loxP and loxP511 sites in the backbone so that undesired cre recombination would not occur. The purified BAC was injected into fertilized eggs to produce to founder lines with one being analyzed in detail. (J:180432)
 Tissue activity of this recombinase allele
Driver: Phox2b (mouse)
Summary of all recombinase alleles driven by Phox2b.

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Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Original:  J:180432 D'Autreaux F, et al., Homeoprotein Phox2b commands a somatic-to-visceral switch in cranial sensory pathways. Proc Natl Acad Sci U S A. 2011 Dec 13;108(50):20018-23
All:  11 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
MGI 6.24
The Jackson Laboratory