Cdh5^{tm4(Cdh5/Fkbp,Ptprb/Mtor*)Dvst}
Targeted Allele Detail

Summary

• Symbol: Cdh5^{tm4(Cdh5/Fkbp,Ptprb/Mtor*)Dvst}
• Name: cadherin 5; targeted mutation 4, Dietmar Vestweber
• MGI ID: MGI:5305106
• Synonyms: VE-cadherin FKBP/VE-PTP FRB*
• Gene: Cdh5 Location: Chr8:104828257-104871143 bp, + strand Genetic Position: Chr8, 53.04 cM
• Alliance: Cdh5^{tm4(Cdh5/Fkbp,Ptprb/Mtor*)Dvst} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:178759
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Inserted expressed sequence, RMCE-ready)
• Mutations: Insertion, Intragenic deletion
• Cdh5^{tm4(Cdh5/Fkbp,Ptprb/Mtor*)Dvst} expresses 4 genes
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Cdh5 (MGI:105057)
  mouse	Fkbp1a (MGI:95541)
  mouse	Mtor (MGI:1928394)
  mouse	Ptprb (MGI:97809)

• Mutation details: A floxed cassette containing the rapamycin binding domain of the FK 506 binding protein fused to the C terminus of Cdh5, an IRES, a mutated form of the rapamycin binding domain of mammalian target of rapamycin (FRB*), containing an HA-tag at its C terminus, fused to the C terminus of Ptprb, and an FRT flanked hygromycin gene was inserted via recombinase mediated cassette exchange using Cdh5^tm1Dvst. In lungs from homozygous mice wild-type protein is not expressed and expression of the mutant Cdh5/Fkbp protein is detected at levels similar to wild-type protein in control mice. Expression of the mutant Ptprb/Mtor* protein is about 30% of the level of expression of the endogenous protein in lungs from homozygous mice. Dissociation of heterodimers of the two mutant proteins is impaired in the presence of rapalog. (J:178759)

Generation of the Cdh5^{tm4(Cdh5/Fkbp,Ptprb/Mtor*)Dvst} allele

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cdh5 Mutation: 53 strains or lines available

Notes

This allele was generated from Cdh5^tm1Dvst.

References

• Original: J:178759 Broermann A, et al., Dissociation of VE-PTP from VE-cadherin is required for leukocyte extravasation and for VEGF-induced vascular permeability in vivo. J Exp Med. 2011 Nov 21;208(12):2393-401
• All: 3 reference(s)