Hprt1tm105(Ple39-EGFP)Ems
Targeted Allele Detail
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Symbol: |
Hprt1tm105(Ple39-EGFP)Ems |
Name: |
hypoxanthine phosphoribosyltransferase 1; targeted mutation 105, Elizabeth M Simpson |
MGI ID: |
MGI:5303187 |
Gene: |
Hprt1 Location: ChrX:52077014-52110536 bp, + strand Genetic Position: ChrX, 29.31 cM, cytoband A6
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Allele Type: |
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Targeted (Reporter) |
Mutation: |
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Insertion
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Mutation details: The Ple39-EGFP plasmid (pEMS1156) was created by inserting the Ple39 MiniPromoter (derived from specific promoter/enhancer/regulatory regions of the human corticotropin releasing hormone (CRH) gene) into the multiple cloning site of the pEMS1306 plasmid vector backbone. This placed the Ple39 MiniPromoter upstream of a minimal F5 mutant-frt site, a Kozak consensus sequence, an enhanced green fluorescent protein (EGFP; with mutated TAA to TTA stop), a tetracysteine residue, a nuclear localization signal, a minimal wildtype frt site, an SV40 early polyA signal, a human HPRT complementary sequence (containing exon 1, intron 1, exon 2, and part of intron 2), a mouse 3' Hprt homology arm, an I-Sce linearization cut site, and a mouse 5' Hprt homology arm. This Ple39-EGFP construct was targeted as a single copy knock-in to the Hprtb-m3 mutant locus on the X Chromosome via electroporation into mEMS1218 embryonic stem cells.
(J:164356)
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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Carrying any Hprt1 Mutation: |
1274 strains or lines available
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Original: |
J:164356 Portales-Casamar E, et al., A regulatory toolbox of MiniPromoters to drive selective expression in the brain. Proc Natl Acad Sci U S A. 2010 Sep 21;107(38):16589-94 |
All: |
1 reference(s) |
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