Pclaf^tm1.1Lkd
Targeted Allele Detail

Summary

• Symbol: Pclaf^tm1.1Lkd
• Name: PCNA clamp associated factor; targeted mutation 1.1, Lisa K Denzin
• MGI ID: MGI:5302414
• Synonyms: Paf^-
• Gene: Pclaf Location: Chr9:65797519-65810548 bp, + strand Genetic Position: Chr9, 35.58 cM
• Alliance: Pclaf^tm1.1Lkd page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:177782
• Parent Cell Line: CY2.4 (ES Cell)
• Strain of Origin: B6(Cg)-Tyr^c-2J

Mutation description

Allele Type: Targeted (Null/knockout) Mutations: Insertion, Intragenic deletion Mutation details: An FRT-flanked neo cassette with a 5' loxP site was inserted upstream of the translation initiation site in exon 1. An additional loxP site was inserted downstream of exon 2. Cre-mediated recombination removed the neo cassette, 3' portion of exon 1 downstream of the translation initiation codon, intron 1, and exon 2. Western blot analysis confirmed the absence of protein expression in thymocytes. (J:177782)

Generation of the Pclaf^tm1.1Lkd allele

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pclaf Mutation: 8 strains or lines available

References

• Original: J:177782 Amrani YM, et al., The Paf oncogene is essential for hematopoietic stem cell function and development. J Exp Med. 2011 Aug 29;208(9):1757-65
• All: 1 reference(s)