Cd79a^tm1.1Rsky
Targeted Allele Detail

Summary

• Symbol: Cd79a^tm1.1Rsky
• Name: CD79A antigen (immunoglobulin-associated alpha); targeted mutation 1.1, Klaus Rajewsky
• MGI ID: MGI:5301876
• Synonyms: Igalpha^SATV
• Gene: Cd79a Location: Chr7:24596922-24601283 bp, + strand Genetic Position: Chr7, 13.49 cM
• Alliance: Cd79a^tm1.1Rsky page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:179254
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: Exon 5, coding for part of the Ig-alpha cytoplasmic domain, was targeted with T>G and AC>GT nucleotide substitutions resulting in the amino acid substitutions of serine with alanine at positions 191 and 197 (p.S191A, p.S197A) and threonine with valine at position 203 (p.T203V), respectively. The targeting vector consisted of the wild-type gene with a loxP site inserted into intron 3, a loxP site flanked neomycin resistance gene cassette downstream of the gene and a copy of the 3' end of the gene starting in intron 3 and including a mutated exon 5. Subsequent cre-mediated recombination deleted the wild-type exons 4 and 5 and the neo cassette, leaving a complete gene coding for the mutated peptide. (J:179254)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cd79a Mutation: 22 strains or lines available

References

• Original: J:179254 Patterson HC, et al., Cytoplasmic Ig alpha serine/threonines fine-tune Ig alpha tyrosine phosphorylation and limit bone marrow plasma cell formation. J Immunol. 2011 Sep 15;187(6):2853-8
• All: 1 reference(s)