Tg(tetO-GCaMP2)12IRyu
Transgene Detail

Summary

• Symbol: Tg(tetO-GCaMP2)12IRyu
• Name: transgene insertion 12I, Ron Yu
• MGI ID: MGI:5301601
• Transgene: Tg(tetO-GCaMP2)12IRyu Location: unknown
• Alliance: Tg(tetO-GCaMP2)12IRyu page

Transgene origin

• Strain of Origin: (CBA x FVB)F1

Transgene description

• Transgene Type: Transgenic (Inducible, Reporter)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Mutation details: This transgene contains a calcium-sensing molecule, GCaMP2, under the transcriptional control of the tetracycline-responsive promoter element (tetO). GCaMP2 consists of, from 5' to 3', a 13-residue peptide of myosin light chain kinase (M13), a circularly permutated eGFP interrupted at residue 145, calmodulin (CaM), and a polyA recognition sequence. Mutations resulting in increased brightness (D180Y and V93I), thermal stability (V163A, and S175G), and GFP dimerization prevention (A206K) were introduced. (J:157448, J:179273)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:179273 Yu R, Direct Data Submission 2012/01/17. MGI Direct Data Submission. 2012
• All: 10 reference(s)