Mecom^tm2.2Aspe
Targeted Allele Detail

Summary

• Symbol: Mecom^tm2.2Aspe
• Name: MDS1 and EVI1 complex locus; targeted mutation 2.2, Archibald Perkins
• MGI ID: MGI:5301308
• Synonyms: Evi1^ko3
• Gene: Mecom Location: Chr3:30005445-30563937 bp, - strand Genetic Position: Chr3, 12.66 cM
• Alliance: Mecom^tm2.2Aspe page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:178429
• Parent Cell Line: S1B6 (ES Cell)
• Strain of Origin: 129S1/SvImJ or C57BL/6J

Mutation description

• Allele Type: Targeted (Hypomorph)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Cre-mediated recombination removed exon 3. RT-qPCR confirmed absence of transcripts encoding exons 2 and 3, however transcripts encoding exons 13 and 14 are produced. Western blot analysis indicates that protein is produced and that this is a hypmorphic allele resulting from expression of an N-terminally truncated protein initiated in exon 4. (J:178429, J:213829)

Expression

In Structures Affected by this Mutation:

9 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mecom Mutation: 82 strains or lines available

References

• Original: J:178429 Zhang Y, et al., PR-domain-containing Mds1-Evi1 is critical for long-term hematopoietic stem cell function. Blood. 2011 Oct 6;118(14):3853-61
• All: 2 reference(s)