Tg(CD2-Akt1*)4-1Psoh
Transgene Detail

Summary

• Symbol: Tg(CD2-Akt1*)4-1Psoh
• Name: transgene insertion 4-1, Pamela Ohashi
• MGI ID: MGI:5300976
• Synonyms: hCD2-gag-PKB
• Transgene: Tg(CD2-Akt1*)4-1Psoh Location: unknown
• Alliance: Tg(CD2-Akt1*)4-1Psoh page

Transgene origin

• Strain of Origin: (CBA/J x C57BL/6)F2

Transgene description

• Transgene Type: Transgenic (Constitutively active, Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: Transgenic mice overexpress a constitutively active form of PKB (gag-PKB (Akt1) in T lymphocytes under control of the human CD2 promoter and locus control region (LCR). The gag-PKB-encoded protein is equivalent to the oncogenic form of PKB, v-akt, and consists of three parts: an in frame fusion of the viral gag protein, a 21-amino acid peptide linker derived primarily from the 5'untranslated region of the endogenous Akt1 gene locus, and Akt1. An NH2-terminal glycine residue of the gag portion of the chimeric protein serves as a site for myristylation. This myristyl group preferentially targets the gag-PKB fusion protein to the cell membrane where it becomes constitutively activated by upstream kinases. Two individual founder lines were established, 5-4 and 4-1. (J:62176)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:62176 Jones RG, et al., Protein kinase B regulates T lymphocyte survival, nuclear factor kappaB activation, and Bcl-X(L) levels in vivo. J Exp Med. 2000 May 15;191(10):1721-34
• All: 4 reference(s)