Tg(Dmp1-cre/ERT2)D77Pdp
Transgene Detail
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| Symbol: |
Tg(Dmp1-cre/ERT2)D77Pdp |
| Name: |
transgene insertion D77, Paola Divieti Pajevic |
| MGI ID: |
MGI:5297120 |
| Synonyms: |
Dmp1-CreERt2 |
| Transgene: |
Tg(Dmp1-cre/ERT2)D77Pdp Location: unknown
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| Alliance: |
Tg(Dmp1-cre/ERT2)D77Pdp page
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| Transgene Type: |
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Transgenic (Inducible, Recombinase) |
| Inducer: |
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tamoxifen |
| Mutation: |
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Insertion
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Tg(Dmp1-cre/ERT2)D77Pdp expression driven by
1 gene
Transgene expression driven by:
| Organism |
Driver Gene |
Note |
| Not Specified |
Dmp1 |
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Mutation details: A fragment (-9624 - +4439) of the Dmp1 gene containing a 9624 bp promoter region, the 95 bp exon 1, 4326 bp intron 1 and 17 bp of the initial non-coding region of exon 2 was cloned into a vector in front of the cre/ERT2 cDNA. The linearized vector was injected into B6C3F1 pronuclei. 14 pups that were positive for cre integration were identified and six independent founders were crossed to wild-type B6 mice. Two founders demonstrated penetrant excision of the STOP cassette when crossed to Rosa26-lacZ reporter mice, and were analyzed in greater detail. Two 10kb-Dmp1-Cre-ERT2 transgenic founder lines (D77 and 0022) were identified to have tamoxifen-inducible Cre recombinase activity in both osteocytes and mature osteoblasts. The two lines were similar with the only difference that line D77 had greater expression in mature osteoblasts.
(J:170229, J:235198)
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Activity: |
 Tissue activity of this recombinase allele
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Driver:
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Dmp1
(species not specified)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Original: |
J:235198 Kim SW, et al., Intermittent parathyroid hormone administration converts quiescent lining cells to active osteoblasts. J Bone Miner Res. 2012 Oct;27(10):2075-84 |
| All: |
10 reference(s) |
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Analysis Tools
