Lgr5^{tm1(cre/ERT2)Fjs}
Targeted Allele Detail

Summary

• Symbol: Lgr5^{tm1(cre/ERT2)Fjs}
• Name: leucine rich repeat containing G protein coupled receptor 5; targeted mutation 1, Frederick J de Sauvage
• MGI ID: MGI:5294796
• Synonyms: Lgr5^CreER
• Gene: Lgr5 Location: Chr10:115286219-115423685 bp, - strand Genetic Position: Chr10, 63.51 cM
• Alliance: Lgr5^{tm1(cre/ERT2)Fjs} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:177145
• Parent Cell Line: C2 (Nagy) (ES Cell)
• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Targeted (Inducible, Null/knockout, Recombinase)
• Inducer: tamoxifen
• Mutations: Insertion, Intragenic deletion
• Lgr5^{tm1(cre/ERT2)Fjs} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Lgr5 (MGI:1341817)

• Mutation details: A targeting vector was generated so that a dsRed-IRES-cre/ERT2-pA-Frt-neo-Frt cassette was inserted immediately after the ATG codon in exon 1 of the Lgr5 locus (deleting the remainder of exon 1 and splice donor site in intron 1 - a212 bp deletion) by homologous recombination in C2 ES cells. Correctly targeted clones were transfected with an Flpe plasmid to remove the neo cassette. (J:177145)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Lgr5 (mouse) Summary of all recombinase alleles driven by Lgr5. Alliance mouse Lgr5 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Lgr5 Mutation: 58 strains or lines available

References

• Original: J:177145 Tian H, et al., A reserve stem cell population in small intestine renders Lgr5-positive cells dispensable. Nature. 2011 Oct 13;478(7368):255-9
• All: 2 reference(s)