Vav3^{Idd18.1-C57BL/6J}
QTL Variant Detail

Summary

• QTL variant: Vav3^{Idd18.1-C57BL/6J}
• Name: vav 3 oncogene; C57BL/6J
• MGI ID: MGI:5288361
• Synonyms: Idd18.1^C57BL/6
• QTL: Vav3 Location: Chr3:109247969-109593014 bp, + strand Genetic Position: Chr3, 48.13 cM, cytoband G1

Variant origin

• Strain of Specimen: C57BL/6J

Variant description

• Allele Type: QTL
• Mutation: Not Applicable
• Mutation details: No coding sequence polymorphisms exist between NOD and C57BL/6 mice. However, 138 SNPs are found between intron 20 and the 3' untranslated region. (J:160442)

Notes

Candidate Genes

J:101473

Microarray gene chip technology was used to identify differentially expressed candidate genes for the arthritis QTL on mouse Chromosome 3 named Cia5. The Cia5 QTL microarray chip contains 163 genes found between 95.3 Mb and 109.7 Mb on mouse Chromosome 3. (This region spans markers D3Mit101 to D3Mit103.) Gene expression in lymph nodes, spleen, and paws was compared between the resistant Cia5 congenic and susceptible B10.RIII background strain. Animals were immunized with 100 ug of bovine CII at 12 weeks of age. Gene expression was evaluated at 0, 6, and 12 days after immunization. A total of 26 genes in the Cia5 locus were found to have significant expression differences (P<0.05) between the congenic and B10.RIII. These genes were found in 2 clusters corresponding to Cia21 and Cia22. Some notable candidate genes are described below.

Chi3l3 (50.5 cM; 105.9 Mb) is downregulated in congenic animals in spleen (all time points) and lymph nodes (day 6).

Vav3 (109.1 Mb) is upregulated in congenic animals in spleen (all time points), lymph nodes (day 0), and paws (days 6 and 12).

Gpr89 (96.3 Mb) is upregulated in congenic animals in spleen (all time points) and lymph nodes (day 0).

Ptpn22 (103.2 Mb) is downregulated in congenic animals in lymph nodes (day 0).

Cd53 (50.5 cM; 106.5 Mb) is upregulated in B10.RIII in spleen (day 12) and lymph nodes (day 0).

Gstm1 (107.8 Mb) is upregulated in B10.RIII in spleen and lymph nodes at day 0, but is downregulated after immunization.

References

• Original: J:160442 Fraser HI, et al., Nonobese diabetic congenic strain analysis of autoimmune diabetes reveals genetic complexity of the Idd18 locus and identifies Vav3 as a candidate gene. J Immunol. 2010 May 1;184(9):5075-84
• All: 1 reference(s)