Eif2a^{Gt(IST13504C3)Tigm}
Gene trapped Allele Detail

Summary

• Symbol: Eif2a^{Gt(IST13504C3)Tigm}
• Name: eukaryotic translation initiation factor 2A; gene trap IST13504C3, Texas A&M Institute for Genomic Medicine
• MGI ID: MGI:5170065
• Gene: Eif2a Location: Chr3:58433252-58464922 bp, + strand Genetic Position: Chr3, 28.58 cM
• Alliance: Eif2a^{Gt(IST13504C3)Tigm} page

Mutation origin

• Mutant Cell Line: IST13504C3
• Germline Transmission: Earliest citation of germline transmission: J:204812
• Parent Cell Line: Lex3.13 (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Gene trapped (Null/knockout)
• Mutation: Insertion of gene trap vector
• Mutation details: A retroviral insertion of a gene trap vector occurred in intron 1. RT-PCR and western blot analysis confirmed the absence of mRNA and protein expression in heart, brain, lung, liver, kidney and pancreas from homozygous mice. (J:250411)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  IST13504C3BBF1	JM233656 ; (MGI Seq Detail)	inverse PCR	Chr3:58433337-58433543 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  43 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 1 line available
• Carrying any Eif2a Mutation: 48 strains or lines available

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 6 reference(s)