Tg(Eno2-Glrb)416Wha
Transgene Detail

Summary

• Symbol: Tg(Eno2-Glrb)416Wha
• Name: transgene insertion 416, Hans Weiher
• MGI ID: MGI:5086008
• Synonyms: rat GlyR beta transgene, tg 416, Tg(NSE-Glrb)416
• Transgene: Tg(Eno2-Glrb)416Wha Location: unknown
• Alliance: Tg(Eno2-Glrb)416Wha page

Transgene origin

• Strain of Origin: (C57BL/6 x DBA/2)F1 x DBA/2

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Eno2-Glrb)416Wha expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  rat

  Glrb (25456)

  Glrb	(MGI:95751)

• Mutation details: The transgene contains the promoter of the rat enolase 2, gamma neuronal gene driving expression of a cDNA encoding the beta subunit of the rat glycine receptor, which is followed by the SV40 small T-antigen splice and polyadenylation signals. Brains of heterozygous transgenic mice of line 416 on a homozygous Glrb^spa background contain transgene-derived mRNA at ~16% and total exon 4- and 5-containing mRNA at ~25% of the level of endogenous Glrb mRNA present in brains of wild-type mice, as visualized by northern blot analysis. In-situ hybridization demonstrates widespread expression in the brain, including the brain stem, and particularly in the facial nucleus and superior olivary complex. Western blot analysis demonstrates presence of membrane-bound glycine receptor subunit alpha, a proxy for the beta subunit. (J:32212)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

Notes

Four transgenic founder lines were derived. Mice of line 416. which exhibited the highest expression, demonstrated complete rescue of the neurologic and reproductive phenotypes of Glrb^spa (J:32212).

References

• Original: J:32212 Hartenstein B, et al., Low level expression of glycine receptor beta subunit transgene is sufficient for phenotype correction in spastic mice. EMBO J. 1996 Mar 15;15(6):1275-82
• All: 1 reference(s)