Notch1^{tm5(cre/ERT2)Rko}
Targeted Allele Detail

Summary

• Symbol: Notch1^{tm5(cre/ERT2)Rko}
• Name: notch 1; targeted mutation 5, Raphael Kopan
• MGI ID: MGI:5009237
• Synonyms: N1::CreERT2, NIP1::CreER^T2, Notch1::CreERT2
• Gene: Notch1 Location: Chr2:26347914-26393834 bp, - strand Genetic Position: Chr2, 18.91 cM
• Alliance: Notch1^{tm5(cre/ERT2)Rko} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:220446
• Parent Cell Line: B6/BLU (ES Cell)
• Strain of Origin: C57BL/6NTac-Tg(HBB-lacZ)ALey/Ley

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutations: Insertion, Intragenic deletion
• Notch1^{tm5(cre/ERT2)Rko} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Notch1 (MGI:97363)

• Mutation details: The targeting construct was designed to replace exons 29-34 (encoding the intracellular domain) with a CreER^T2 sequence and nuclear localization signal, followed immediately downstream by a C-terminal 6xMyc epitope tag, 2xSV40 polyadenylation sequence, and frt-flanked Pgk1/EM7 promoter-driven neomycin selection cassette. The CreER fusion gene (Cre-ER^T2) is Cre recombinase fused to a G400V/M543A/L544A triple mutation of the human estrogen receptor ligand binding domain. (J:172930)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Notch1 (mouse) Summary of all recombinase alleles driven by Notch1. Comparative matrix view of recombinase activities Alliance mouse Notch1 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Notch1 Mutation: 115 strains or lines available

References

• Original: J:172930 Liu Z, et al., Rapid identification of homologous recombinants and determination of gene copy number with reference/query pyrosequencing (RQPS). Genome Res. 2009 Nov;19(11):2081-9
• All: 7 reference(s)