Prap1^tm1Lex
Targeted Allele Detail

Summary

• Symbol: Prap1^tm1Lex
• Name: proline-rich acidic protein 1; targeted mutation 1, Lexicon Pharmaceuticals
• MGI ID: MGI:5007296
• Synonyms: Prap1^-
• Gene: Prap1 Location: Chr7:139673308-139677113 bp, + strand Genetic Position: Chr7, 85.1 cM, cytoband F5
• Alliance: Prap1^tm1Lex page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:171883
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S5/SvEvBrd
• Project Collection: Lexicon

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: The Prap1 gene consists of 5 exons. Coding exons 1 through 5 were targeted (NCBI accession NM_009475.1) by homologous recombination. Disruption of the target gene was confirmed by Southern hybridization analysis. Western blot analysis using novel PRAP1 rabbit antisera demonstrated the absence of protein expression in small intestine and uterine whole tissue from homozygous mutant mice. Immunofluorescence staining confirmed the absence of detectable signal in duodenal tissue. (J:171883, J:304152)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Prap1 Mutation: 14 strains or lines available

Notes

See also, data as provided by Lexicon Genetics, Inc.

References

• Original: J:161922 Tang T, et al., A mouse knockout library for secreted and transmembrane proteins. Nat Biotechnol. 2010 Jul;28(7):749-55
• All: 3 reference(s)