Nek7^tm1Mot
Targeted Allele Detail

Summary

• Symbol: Nek7^tm1Mot
• Name: NIMA (never in mitosis gene a)-related expressed kinase 7; targeted mutation 1, Benny Motro
• MGI ID: MGI:4999797
• Gene: Nek7 Location: Chr1:138411575-138547481 bp, - strand Genetic Position: Chr1, 60.82 cM
• Alliance: Nek7^tm1Mot page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:168166
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Most of exons 3 and 4 of the gene is replaced with b-galactosidase (b-gal)-Neo resistance cassette, which deletes 1630 bp and insert premature termination of Nek7 coding at amino-acid 75 but leaving intact the splice acceptor site of exon 3 and the splice donor site of exon 4. Western blot confirmed absence of protein product in cultured MEF. This is a null allele. (J:168166)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nek7 Mutation: 39 strains or lines available

References

• Original: J:168166 Salem H, et al., Nek7 kinase targeting leads to early mortality, cytokinesis disturbance and polyploidy. Oncogene. 2010 Jul 15;29(28):4046-57
• All: 3 reference(s)