Tg(Sox10-GFP,-DTA)1Wdr
Transgene Detail

Summary

• Symbol: Tg(Sox10-GFP,-DTA)1Wdr
• Name: transgene insertion 1, William D Richardson
• MGI ID: MGI:4999728
• Synonyms: Sox10-DTA, Sox10-lox-GFP-STOP-lox-DTA
• Transgene: Tg(Sox10-GFP,-DTA)1Wdr Location: unknown
• Alliance: Tg(Sox10-GFP,-DTA)1Wdr page

Transgene origin

• Strain of Origin: Not Specified

Transgene description

• Transgene Type: Transgenic (Reporter)
• Mutation: Insertion
• Mutation details: A 120 kb fragment of a genomic PAC containing the entire Sox10 locus was used to generate the transgene. The region spanning exons 3-5 of Sox10 was replaced by homologous recombination in bacteria with a loxP-flanked eGFP polyA cassette followed by sequences encoding an attenuated G383A version of the diphtheria toxin, subunit A (DTA). An Frt-flanked Cm cassette was included for selection purposes, and was removed by transient activation of Flp in bacterial cells. In presence of cre recombinase, the reporter cassette is excised, allowing expression of DTA and ablation of the expressing cells. (J:105334)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:105334 Kessaris N, et al., Competing waves of oligodendrocytes in the forebrain and postnatal elimination of an embryonic lineage. Nat Neurosci. 2006 Feb;9(2):173-9
• All: 8 reference(s)