Aldob^{tm1(KOMP)Vlcg}
Targeted Allele Detail

Summary

• Symbol: Aldob^{tm1(KOMP)Vlcg}
• Name: aldolase B, fructose-bisphosphate; targeted mutation 1, Velocigene
• MGI ID: MGI:4951216
• Synonyms: Aldo2^-
• Gene: Aldob Location: Chr4:49535995-49549546 bp, - strand Genetic Position: Chr4, 26.57 cM
• Alliance: Aldob^{tm1(KOMP)Vlcg} page
• IMPC: Aldob gene page

Mutation origin

• Mutant Cell Lines: 15350A-A6, 15350B-A9, 15350B-F10, 15350C-G4
• Germline Transmission: Earliest citation of germline transmission: J:204812
• Parent Cell Line: VGB6 (ES Cell)
• Strain of Origin: C57BL/6NTac
• Project Collection: KOMP-Regeneron

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: ZEN-Ub1
• Mutation details: The insertion of Velocigene cassette ZEN-Ub1 created a deletion of size 8162bp between positions 49536744-49544905 of Chromosome 4 (Build GRCm39). The cassette replaced 3' of exon 2 through 5' of exon 9. Enzyme activity assays of liver lysates revealed that activity toward fructose 1-phosphate was undetectable, and resulted in a change in the fructose 1,6-bisphosphate:fructose 1-phosphate activity ratio from 1.7 in wild-type mice to over 10 in homozygous mutant mice, indicating that any remaining aldolase activity was due to aldolase A. (J:136110, J:220330)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 1 line available
• Carrying any Aldob Mutation: 22 strains or lines available

Notes

Cell line 15350B-F10 was successfully used to make chimeric mice. Germline transmission was accomplished. Cell line 15350A-A6 was not successfully used to make chimeric mice. J:220330

References

• Original: J:136110 Velocigene, Alleles produced for the KOMP project by Velocigene (Regeneron Pharmaceuticals). MGI Direct Data Submission. 2008
• All: 4 reference(s)