Ubqln3^{tm1(KOMP)Vlcg}
Targeted Allele Detail

Summary

• Symbol: Ubqln3^{tm1(KOMP)Vlcg}
• Name: ubiquilin 3; targeted mutation 1, Velocigene
• MGI ID: MGI:4951160
• Gene: Ubqln3 Location: Chr7:103789830-103792486 bp, - strand Genetic Position: Chr7, 54.85 cM
• Alliance: Ubqln3^{tm1(KOMP)Vlcg} page
• IMPC: Ubqln3 gene page

Mutation origin

• Mutant Cell Lines: 11472B-B1, 11472B-C9, 11472B-G1, 11472B-H12, 11472C-E4, 11472C-G12
• Germline Transmission: Earliest citation of germline transmission: J:244165
• Parent Cell Line: VGB6 (ES Cell)
• Strain of Origin: C57BL/6NTac
• Project Collection: KOMP-Regeneron

Mutation description

Allele Type: Targeted (Null/knockout, Reporter) Mutations: Insertion, Intragenic deletion Vector: ZEN-Ub1 Mutation details: The insertion of Velocigene cassette ZEN-Ub1 created a deletion of size 2000bp between positions 103790086-103792085 of Chromosome 7 (Build GRCm39). Exon 2 was replaced with a lacZ reporter and a floxed neomycin resistance cassette. qRT-PCR and Western blot analysis confirmed complete loss of mRNA and protein expression in the testes of adult homozygotes. (J:136110, J:244165)

Generation of Ubqln3^tm1(KOMP)Vlcg allele

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 1 line available
• Carrying any Ubqln3 Mutation: 40 strains or lines available

References

• Original: J:136110 Velocigene, Alleles produced for the KOMP project by Velocigene (Regeneron Pharmaceuticals). MGI Direct Data Submission. 2008
• All: 2 reference(s)