Prkdc^tm1.1Bpcc
Targeted Allele Detail

Summary

• Symbol: Prkdc^tm1.1Bpcc
• Name: protein kinase, DNA activated, catalytic polypeptide; targeted mutation 1.1, Benjamin P C Chen
• MGI ID: MGI:4950416
• Synonyms: DNA-PKcs^3A
• Gene: Prkdc Location: Chr16:15455730-15660099 bp, + strand Genetic Position: Chr16, 10.09 cM, cytoband B1
• Alliance: Prkdc^tm1.1Bpcc page

Activation of p53 in the intestinal crypt and epidermis in Prkdc^tm1.1Bpcc/ Prkdc^tm1.1Bpcc (DNA-PKcs^3A/3A) mice

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:171351
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted
• Mutation: Nucleotide substitutions
• Mutation details: Three alanine substitutions at Thr2605, Thr2634, and Thr2643 in exon 58 and a self-excising ACN cassette were inserted via homologous recombination. The ACN cassette was removed by cre mediated recombination in the male germline leaving a single loxP site in intron 58. Analysis of whole cell lysates of homozygous MEFs indicates protein expression is not significantly different from the wild-type allele. Kinase activity of the mutant allele is not significantly different from the wild-type allele. The 3 alanine substitutions are in a threonine phosphorylation cluster. (J:171351)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Prkdc Mutation: 408 strains or lines available

References

• Original: J:171351 Zhang S, et al., Congenital bone marrow failure in DNA-PKcs mutant mice associated with deficiencies in DNA repair. J Cell Biol. 2011 Apr 18;193(2):295-305
• All: 1 reference(s)