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Tg(tetO-TPR/MET,-EGFP)12Tcre
Transgene Detail
Summary
Symbol: Tg(tetO-TPR/MET,-EGFP)12Tcre
Name: transgene insertion 12, Tiziana Crepaldi
MGI ID: MGI:4949465
Synonyms: Tg(TPRMET-tetO-GFP)12Tcre, Tpr-Met-TetO-GFP, Tpr-Met-TRE-GFP
Transgene: Tg(tetO-TPR/MET,-EGFP)12Tcre  Location: unknown  
Alliance: Tg(tetO-TPR/MET,-EGFP)12Tcre page
Transgene
origin
Strain of Origin:  FVB
Transgene
description
Transgene Type:    Transgenic (Inducible, Inserted expressed sequence, Reporter)
Inducer:    doxycycline/tetracycline
Mutation:    Insertion
 
Mutation detailsA bi-directional tetracycline response element (TetO) drives expression of an enhanced green fluorescent protein reporter gene (EGFP) and of a cDNA encoding the human TRP-MET oncoprotein, in which the N-terminal of TPR (translocation promoter region), containing a pair of dimerization sites, is joined to the tyrosine kinase domain of the MET proto-oncogene, which is the receptor for hepatocyte growth factor. Crossing mice bearing this transgene with mice that express the tetracycline transactivator (tTA) or reverse transactivator (rtTA) protein under the control of a tissue-specific promoter permits regulation by tetracycline withdrawal/administration of the expression of TRP-MET and of the GFP reporter in the progeny. (J:120883)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Disease models
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Expression
In Structures Affected by this Mutation: 8 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Notes
Multiple founders whose fibroblasts expressed EGFP when infected with a tTA-expressing lentivirus under a ubiquitous promoter were used to establish mouse lines. Founder #12 has two independent insertions which segregate from one another. Sublines #12.2 and #12.9 were used in the reported studies. (J:120883)
References
Original:  J:120883 Crepaldi T, et al., Conditional activation of MET in differentiated skeletal muscle induces atrophy. J Biol Chem. 2007 Mar 2;282(9):6812-22
All:  6 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
01/24/2023
MGI 6.22
The Jackson Laboratory