Ch25h^tm1Rus
Targeted Allele Detail

Summary

• Symbol: Ch25h^tm1Rus
• Name: cholesterol 25-hydroxylase; targeted mutation 1, David W Russell
• MGI ID: MGI:4947407
• Gene: Ch25h Location: Chr19:34451183-34452548 bp, - strand Genetic Position: Chr19, 29.69 cM
• Alliance: Ch25h^tm1Rus page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:170807
• Parent Cell Line: SM1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The intron-less gene was deleted in its entirety and replaced by the PolII neo pA expression cassette. The absence of protein expression was confirmed by western blot analysis on lung and liver extracts after Kdo2-Lipid A induction. (J:170807)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Ch25h Mutation: 20 strains or lines available

References

• Original: J:170807 Bauman DR, et al., 25-Hydroxycholesterol secreted by macrophages in response to Toll-like receptor activation suppresses immunoglobulin A production. Proc Natl Acad Sci U S A. 2009 Sep 29;106(39):16764-9
• All: 36 reference(s)