Tg(Umod-cre)1Dek
Transgene Detail

Summary

• Symbol: Tg(Umod-cre)1Dek
• Name: transgene insertion 1, Donald E Kohan
• MGI ID: MGI:4947390
• Synonyms: line 1 THP-CreTag, THP-CreTag
• Transgene: Tg(Umod-cre)1Dek Location: unknown
• Alliance: Tg(Umod-cre)1Dek page

Transgene origin

• Strain of Origin: C57BL/6 x CBA

Transgene description

• Transgene Type: Transgenic (Recombinase)
• Mutation: Insertion
• Tg(Umod-cre)1Dek expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Umod (MGI:102674)

• Mutation details: A 3.7 kb fragment of the murine Tamms-Horsfall protein gene (THP; official name - uromodulin, Umod) was identified and modified to generate the transgene construct. The 3' end of the Umod fragment containing the first non-coding exon and part of the first intron was modified to create a fusion of cre recombinase and the transcriptional start site/exon 1. The cre recombinase sequence includes an 11 amino acid herpes simplex virus (HSV) glycoprotein D epitope tag to facilitate immunodetection of cre protein. The purified construct was microinjected into pronuclei of C57BL/CBA single cell embryos. Eleven founders were obtained, and six showed transgene transmission. Founders were crossed to ROSA-EYFP reporters to evaluate specificity and recombination efficiency. Line 1 showed the strongest reporter activation in the outer medulla of the kidney with negative staining in other organs. Cre (CreTag) mRNA was detected in the kidney and testes, with low expression in the brain. Line 1 was determined to have one copy of the transgene. (J:84256)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Umod (mouse) Summary of all recombinase alleles driven by Umod. Comparative matrix view of recombinase activities Alliance mouse Umod gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:84256 Stricklett PK, et al., Thick ascending limb-specific expression of Cre recombinase. Am J Physiol Renal Physiol. 2003 Jul;285(1):F33-9
• All: 2 reference(s)