Tg(BEST1-cre)1Jdun
Transgene Detail
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Symbol: |
Tg(BEST1-cre)1Jdun |
Name: |
transgene insertion 1, Joshua L Dunaief |
MGI ID: |
MGI:4946605 |
Synonyms: |
BEST1-cre |
Transgene: |
Tg(BEST1-cre)1Jdun Location: unknown Genetic Position: Chr9, Syntenic
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Alliance: |
Tg(BEST1-cre)1Jdun page
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Transgene Type: |
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Transgenic (Recombinase) |
Mutations: |
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Insertion, Intergenic deletion
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Tg(BEST1-cre)1Jdun expression driven by
1 gene
Transgene expression driven by:
Organism |
Driver Gene |
Homolog in Mouse |
Note |
human |
BEST1 (7439) |
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Mutation details: A fragment of the human BEST1 promoter (nucleotides -585 to +38) drives cre recombinase cDNA, the SV40 t-antigen intron, and HSV-TK polyA in the construct. This was purified and microinjected into C57BL/6 zygotes. Six founders were generated with one F2 line that showed the highest percentage of cre immunoreactivity in the RPE was selected for further study. Expression of cre was mosaic, with 50-90% of RPE cells being labeled. This line has a transgene copy number of 20 determined by qPCR analysis (J:170522) or 5 copies (J:240920) inserted about 58 kb upstream of Tmem108 (chr9:103,722,073; NCBI37/mm9) with a 120 bp deletion at the transgene integration site.
(J:170522, J:240920)
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Activity: |
Tissue activity of this recombinase allele
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Driver:
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BEST1
(human)
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View phenotypes and curated references for all genotypes (concatenated display).
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 1 strain available
Cell Lines: 0 lines available
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Original: |
J:170522 Iacovelli J, et al., Generation of Cre Transgenic Mice with Postnatal RPE-Specific Ocular Expression. Invest Ophthalmol Vis Sci. 2011 Mar;52(3):1378-83 |
All: |
39 reference(s) |
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