Tg(Aqp2-cre/ERT2)4Gsc
Transgene Detail

Summary

• Symbol: Tg(Aqp2-cre/ERT2)4Gsc
• Name: transgene insertion 4, Gunther Schutz
• MGI ID: MGI:4941815
• Synonyms: AQP2CreER^T2
• Transgene: Tg(Aqp2-cre/ERT2)4Gsc Location: unknown
• Alliance: Tg(Aqp2-cre/ERT2)4Gsc page

Transgene origin

• Strain of Origin: FVB/N

Transgene description

• Transgene Type: Transgenic (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Tg(Aqp2-cre/ERT2)4Gsc expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Aqp2 (MGI:1096865)

• Mutation details: A genomic fragment of the murine Aqp2 gene in a PAC was modified by homologous recombination in bacterias to put the sequence for a cre/ERT2 fusion protein at the ATG site of the Aqp2 locus. This construct was microinjected into FVB/N pronuclei to produce transgenic animals. 3 lines with 1, 4, and 9 copies of the transgene were established and analyzed for cre expression. The lines show copy number-dependent quantitative differences but have identical expression profiles. The line with 4 copies was chosen for further studies. (J:169670)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Aqp2 (mouse) Summary of all recombinase alleles driven by Aqp2. Alliance mouse Aqp2 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:169670 Ronzaud C, et al., Inducible renal principal cell-specific mineralocorticoid receptor gene inactivation in mice. Am J Physiol Renal Physiol. 2011 Mar;300(3):F756-60
• All: 1 reference(s)