Rad1^tm1.1Jcbs
Targeted Allele Detail

Summary

• Symbol: Rad1^tm1.1Jcbs
• Name: RAD1 checkpoint DNA exonuclease; targeted mutation 1.1, Heinz Jacobs
• MGI ID: MGI:4941376
• Synonyms: Rad1^flox, Rad1^K185R
• Gene: Rad1 Location: Chr15:10486104-10499149 bp, + strand Genetic Position: Chr15, 5.35 cM, cytoband A2
• Alliance: Rad1^tm1.1Jcbs page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:169551
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Conditional ready, Modified isoform(s))
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: Exon 4 was replaced with a loxP site, modified exon 4 contained nucleotide substitutions that result in the the amino acid substitution of arginine for lysine at position 185 (K185R), and FRT-flanked puro cassette with a 3' loxP. Flp-mediated recombination removed the puro cassette. LoxP sites flanking modified exon 4 remain in this allele. (J:169551)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rad1 Mutation: 43 strains or lines available

References

• Original: J:169551 Wit N, et al., Lysine residue 185 of Rad1 is a topological but not a functional counterpart of lysine residue 164 of PCNA. PLoS One. 2011;6(1):e16669
• All: 3 reference(s)