Tg(CAG-XBP1*/Venus)#Miur
Transgene Detail

Summary

• Symbol: Tg(CAG-XBP1*/Venus)#Miur
• Name: transgene insertion, Masayuki Miura
• MGI ID: MGI:4939273
• Synonyms: ERAI, XBP1/deltaDBD-venus
• Transgene: Tg(CAG-XBP1*/Venus)#Miur Location: unknown
• Alliance: Tg(CAG-XBP1*/Venus)#Miur page

Transgene origin

• Strain of Origin: C57BL/6

Transgene description

• Transgene Type: Transgenic (Reporter)
• Mutation: Insertion
• Mutation details: Nucleotides 410-633 of a human XBP1 cDNA including a Kozak sequence and FLAG-tag coding sequence was fused to the venus fluorescent reporter and then placed under the control of the CAG promoter construct in the pCAX expression vector (pCAX is a variant of pCAGG). The XBP1 fragment lacks the DNA binding domain but includes a 26-nt endoplasmic reticulum stress specific intron. Under normal conditions, the mRNA of the fusion gene is not spliced and translation terminates at a stop codon near the junction of XBP1 and venus. During ER stress, the 26 nt intron is spliced out and translation terminates at the stop codon of venus. (J:87202)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:87202 Iwawaki T, et al., A transgenic mouse model for monitoring endoplasmic reticulum stress. Nat Med. 2004 Jan;10(1):98-102
• All: 19 reference(s)