Tg(Slc17a6-icre)1Oki
Transgene Detail

Summary

• Symbol: Tg(Slc17a6-icre)1Oki
• Name: transgene insertion 1, Ole Kiehn
• MGI ID: MGI:4881727
• Synonyms: BAC-Vglut2::Cre, vGlut2^Cre
• Transgene: Tg(Slc17a6-icre)1Oki Location: unknown
• Alliance: Tg(Slc17a6-icre)1Oki page

Transgene origin

• Strain of Origin: C57BL/6

Transgene description

• Transgene Type: Transgenic (Recombinase)
• Mutation: Insertion
• Tg(Slc17a6-icre)1Oki expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Slc17a6 (MGI:2156052)

• Mutation details: Recombineering methods in bacterial cells were used to insert the codon-improved cre open reading frame and FRT-flanked ampicillin resistance gene after the ATG site of the Slc17a6 locus contained in BAC clone RP23-84M15. Insertion of the targeting construct resulting in inactivation of the Slc17a6 gene in the BAC. Flpe recombinase was induced in the bacterial cells and the ampicillin cassette was excised. The purified 201 kb final construct was injected into the pronuclei of C57BL/6 zygotes. Three positive founders were produced. All founders exhibited similar restricted cre expression in the spinal cord, so a line produced from one founder was analyzed further. (J:168055)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Slc17a6 (mouse) Summary of all recombinase alleles driven by Slc17a6. Comparative matrix view of recombinase activities Alliance mouse Slc17a6 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:168055 Borgius L, et al., A transgenic mouse line for molecular genetic analysis of excitatory glutamatergic neurons. Mol Cell Neurosci. 2010 Nov;45(3):245-57
• All: 24 reference(s)