Gja10^{tm2.1(cre)Kwi}
Targeted Allele Detail

Summary

• Symbol: Gja10^{tm2.1(cre)Kwi}
• Name: gap junction protein, alpha 10; targeted mutation 2.1, Klaus Willecke
• MGI ID: MGI:4867556
• Synonyms: Cx57^cre, Gja10>cre, Gja10^tm2(cre)Kwi
• Gene: Gja10 Location: Chr4:32596960-32602760 bp, - strand Genetic Position: Chr4, 14.25 cM
• Alliance: Gja10^{tm2.1(cre)Kwi} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:167075
• Parent Cell Line: HM-1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd-Hprt1^b-m3

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutations: Insertion, Intragenic deletion
• Gja10^{tm2.1(cre)Kwi} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Gja10 (MGI:1339969)

• Mutation details: The part of exon 2 encoding amino acids 1 through 346 was replaced with an FRT flanked DTR-EGFP cassette and a cre recombinase gene witha nuclear localization sequence. Subsequent flp mediated recombination removed the DTR-EGFP cassette and brought the cre recombinase under the control of the endogenous gene promoter. (J:167075, J:186624, J:207456)

Generation of Gja10^{tm2.1(cre)Kwi}

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Gja10 (mouse) Summary of all recombinase alleles driven by Gja10. Comparative matrix view of recombinase activities Alliance mouse Gja10 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gja10 Mutation: 22 strains or lines available

References

• Original: J:167075 Tykocinski LO, et al., Epigenetic regulation of promiscuous gene expression in thymic medullary epithelial cells. Proc Natl Acad Sci U S A. 2010 Nov 9;107(45):19426-31
• All: 5 reference(s)