Cebpa^{tm1.1(cre)Touw}
Targeted Allele Detail

Summary

• Symbol: Cebpa^{tm1.1(cre)Touw}
• Name: CCAAT/enhancer binding protein alpha; targeted mutation 1.1, Ivo P Touw
• MGI ID: MGI:4867437
• Synonyms: Cebpa^Cre
• Gene: Cebpa Location: Chr7:34818718-34821353 bp, + strand Genetic Position: Chr7, 21.02 cM
• Alliance: Cebpa^{tm1.1(cre)Touw} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:166620
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129 x C57BL/6

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutations: Insertion, Intragenic deletion
• Cebpa^{tm1.1(cre)Touw} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Cebpa (MGI:99480)

• Mutation details: A 14.4 kb targeting vector containing the cre recombinase sequence and an Frt-flanked puromycin selectable marker was used to insert cre after the initiation codon of Cebpa, replacing the single Cebpa exon. Correctly targeted 129/Sv/C57BL/6 ES cells were injected into B6 blastocysts to generate chimeras. The chimeras were crossed to FLPeR mice to remove the puromycin cassette. (J:166620)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Cebpa (mouse) Summary of all recombinase alleles driven by Cebpa. Alliance mouse Cebpa gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cebpa Mutation: 16 strains or lines available

References

• Original: J:166620 Wolfler A, et al., Lineage-instructive function of C/EBPalpha in multipotent hematopoietic cells and early thymic progenitors. Blood. 2010 Nov 18;116(20):4116-25
• All: 8 reference(s)