About   Help   FAQ
Supt5tm2a(KOMP)Wtsi
Targeted Allele Detail
Summary
Symbol: Supt5tm2a(KOMP)Wtsi
Name: suppressor of Ty 5, DSIF elongation factor subunit; targeted mutation 2a, Wellcome Trust Sanger Institute
MGI ID: MGI:4840996
Gene: Supt5  Location: Chr7:28014316-28038171 bp, - strand  Genetic Position: Chr7, 16.68 cM
Alliance: Supt5tm2a(KOMP)Wtsi page
IMPC: Supt5 gene page
Mutation
origin
Mutant Cell Lines:  EPD0657_1_A05, EPD0657_1_A06, EPD0657_1_A07, EPD0657_1_A08, EPD0657_1_C07, EPD0657_1_C08, EPD0657_1_D05, EPD0657_1_D06, EPD0657_1_D07, EPD0657_1_D08, EPD0657_1_E05, EPD0657_1_E06, EPD0657_1_E07, EPD0657_1_E08, EPD0657_1_F06, EPD0657_1_F07, EPD0657_1_F08, EPD0657_1_G05, EPD0657_1_G06, EPD0657_1_G07, EPD0657_1_G08, EPD0657_1_H06
Germline Transmission:  Earliest citation of germline transmission: J:204812
Parent Cell Line:  JM8A3.N1 (ES Cell)
Strain of Origin:  C57BL/6N-Atm1Brd
Project Collection: KOMP-CSD
Mutation
description
Allele Type:    Targeted (Conditional ready, Null/knockout, Reporter)
Mutation:    Insertion     Vector: L1L2_Bact_P
 
Mutation detailsThe L1L2_Bact_P cassette was inserted at position 28021165 of Chromosome 7 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 28022484. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:148605, J:173534)
Phenotypes
Loading...
View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 1 anatomical structure(s)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 3 strains available      Cell Lines: 1 line available
Carrying any Supt5 Mutation:  58 strains or lines available
References
Original:  J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009;
All:  5 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support.
last database update
05/28/2024
MGI 6.13
The Jackson Laboratory