Gt(ROSA)26Sor^{tm7(SMO*/YFP)Amc}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm7(SMO*/YFP)Amc}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 7, Andrew P McMahon
• MGI ID: MGI:4839792
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm7(SMO*/YFP)Amc} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:165962
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: mixed

Mutation description

• Allele Type: Targeted (Conditional ready, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm7(SMO*/YFP)Amc} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Smo (MGI:108075)

• Mutation details: A tetracycline regulatory unit and polyadenylation signals were inserted upstream of a tetO and minimal CMV promoter. This was followed by a floxed PGK-neomycin-polyA cassette and then by a cDNA for the human smoothened homolog containing a Trp to Leu mutation in codon 535 and fused to YFP. Expression of the SMO* knock-in occurs only after cre excision of the PGK-neo cassette which can be accomplished by tamoxifen treatment to activate cre expression from the TG(CAG-cre/Esr1*)5Amc transgene carried by the YFP3-1 ES cell line used to create this mutation. (J:165962)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1046 strains or lines available

Notes

YFP3-1 ES cell line = YFP3-1. These cells were derived from embryos resulting from an intercross between Gt(ROSA)26Sor^tm1Sor; Tg(CAG-cre/Esr1*)5Amc male mice and female Gt(ROSA)26Sor^tm1(EYFP)Cos mice. This male ES cell line carries Tg(CAG-cre/Esr1*)5Amc and is heterozygous Gt(ROSA)26Sor^{tm1Sor/tm1(EYFP)Cos} on a background involving 129S4/SvJae, 129X1/SvJ, C57BL/6, CBA, and Swiss Webster.

References

• Original: J:165962 Mao J, et al., An ES cell system for rapid, spatial and temporal analysis of gene function in vitro and in vivo. Nucleic Acids Res. 2005;33(18):e155
• All: 1 reference(s)